Anti-CTCF Antibody

A rabbit polyclonal antibody to CTCF is offered by Antibodies.com as part of their Primary Antibodies range. The reagent is produced in a rabbit host, has polyclonal clonality with an IgG isotype, and is supplied unconjugated. It reacts with Human, Mouse, Rat, and Monkey samples and has been validated for WB, IHC, IF, IP, and ChIP.

According to the manufacturer's specification sheet, the immunogen is a recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1), and the target has a molecular weight of 140 kDa. The product is supplied as a liquid formulated in Phosphate Buffered Saline (pH 7.3) with 50% glycerol and 0.05% Proclin 300, and it is purified by affinity purification. Recommended dilutions are WB 1:500-1:2,000, IHC 1:50-1:200, ICC/IF 1:50-1:200, IP 1:100-1:500, and ChIP 1:50-1:200. The antibody is shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided.