Anti-CTCF Antibody

Raised in rabbit against the CCCTC-binding factor (CTCF), this polyclonal IgG primary antibody from Antibodies.com is validated for western blot and immunohistochemistry applications. CTCF is a highly conserved zinc finger protein recognized for its role as a transcriptional insulator, chromatin boundary element, and master organizer of three-dimensional genome architecture, making it a frequent target in studies of gene regulation, parental imprinting, and higher-order chromatin structure.

This unconjugated reagent is supplied in a 200 µl volume and reacts with human and mouse samples, supporting cross-species investigation of CTCF-mediated processes such as enhancer blocking, X-chromosome inactivation, and topologically associated domain formation. The polyclonal host format and standard IgG isotype allow compatibility with HRP- and fluorophore-conjugated secondary reagents commonly used in immunoblotting and tissue-section imaging protocols.

Researchers working in epigenetics, chromatin biology, or nuclear organization can apply this antibody to characterize CTCF occupancy at boundary regions, monitor changes in chromatin looping under experimental perturbation, or profile insulator function in normal versus disease states. For best results, store refrigerated, protect from light, and aliquot upon first use to limit freeze-thaw cycles and preserve activity across repeated experiments.