Anti-CstF-64 Antibody

This polyclonal antibody from Antibodies.com targets CstF-64, the 64 kDa subunit of the cleavage stimulation factor complex responsible for pre-mRNA 3' end cleavage and polyadenylation in eukaryotic cells. Raised in rabbit and supplied unconjugated in a 100 µL volume, the reagent is validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows and detects endogenous CstF-64 in Human and Mouse samples.

CstF-64 operates as part of the trimeric CstF complex together with CstF-50 and CstF-77, where it binds GU-rich downstream sequence elements and recruits the cleavage and polyadenylation machinery to the nascent transcript. Through this activity, CstF-64 shapes 3' UTR length, influences alternative polyadenylation site selection, and contributes to mRNA stability, nuclear export, and translation efficiency. Researchers investigating gene expression regulation, RNA biology, B-cell receptor diversification, and somatic hypermutation use CstF-64 detection to explore how altered 3' end processing drives cancer progression, immune deficiency, and developmental disorders.

The unconjugated format gives laboratories flexibility to conjugate the antibody to HRP, fluorophores, biotin, or other reporters depending on the experimental design, and compatibility across multiple assay platforms supports both routine sample screening and mechanistic follow-up studies in cell and tissue contexts.