Anti-CSPG4 Antibody

This Rabbit polyclonal antibody, available from Antibodies.com as a primary antibody, recognises CSPG4, also referred to as NG2. It is unconjugated and of the IgG isotype, and is reactive with Human, Mouse and Rat samples. The catalogue entry describes the antibody as validated for WB and IF applications.

The manufacturer datasheet additionally lists validated applications of WB, IHC-P, ICC/IF and ELISA and notes that the reagent is KO Validated. Recommended dilutions are WB: 1:500-1:1,000, IHC-P: 1:50-1:200, and ICC/IF: 1:50-1:200. The immunogen is a recombinant fusion protein corresponding to amino acids 1340-1563 of human NG2, and the antigen species is Human.

The antibody is affinity purified and supplied as a liquid in Phosphate Buffered Saline, pH 7.3, containing 50% Glycerol and 0.05% ProClin 300. The predicted molecular weight is 251 kDa, with a reported molecular weight range of 250 kDa-450 kDa, and the concentration is lot specific. The product is shipped at 4°C and, upon delivery, should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided.