Anti-CRY2 Antibody

Cryptochrome circadian regulator 2 (CRY2) is a flavin adenine dinucleotide-binding photoreceptor of the photolyase/cryptochrome family that acts as a core repressor in the mammalian circadian transcription-translation feedback loop. The Antibodies.com Anti-CRY2 Antibody is a rabbit polyclonal reagent raised against human CRY2, supplied as 100 µl of unconjugated antiserum and validated for Western blot (WB) and immunohistochemistry (IHC) applications on human specimens.

CRY2 partners with PER1 and PER2 to enter the nucleus and inhibit BMAL1-CLOCK-driven transcription of clock-controlled genes, generating the roughly 24-hour cellular rhythm. Beyond circadian regulation, CRY2 participates in DNA damage checkpoint signaling through interactions with TIMELESS and CHK1, and published studies link CRY2 activity to glucagon secretion and glucose homeostasis. The predicted molecular mass of the human CRY2 protein is approximately 67 kDa, with immunoreactive bands typically observed in this region on Western blots of whole-cell lysates.

For long-term stability, aliquot the antiserum into working volumes on first thaw and store at -20 °C to limit freeze-thaw cycles; the antibody remains stable for short-term use on ice during active experiments. Each vial contains 100 µl of single-use reagent suitable for direct labeling or detection with HRP- or fluorophore-conjugated secondary antibodies, supporting studies of circadian gene regulation and DNA damage checkpoint control.