Anti-Crk2 Antibody

This primary antibody from Antibodies.com is a mouse monoclonal reagent raised against the Crk2 adaptor protein, supplied as 100 µl of purified immunoglobulin in a stabilizing buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% sodium azide, and 50% glycerol. The unconjugated antibody recognizes human Crk2 and is validated for Western blot detection of endogenous protein levels in cell lysates and tissue homogenates.

Crk2 belongs to the CRK family of Src homology adaptor proteins, which couple tyrosine-phosphorylated signaling intermediates to downstream effectors through tandem SH2 and SH3 domains. By assembling multi-protein complexes at the cell membrane, the antibody target participates in growth-factor receptor signaling, integrin-mediated adhesion, cytoskeletal remodeling, and cell migration pathways. Researchers studying receptor tyrosine kinase activity, focal adhesion dynamics, and oncogenic transformation frequently probe this adaptor in signaling studies, given its role in linking phosphorylated p130Cas and paxillin networks to effectors such as DOCK180 and Rap1.

The monoclonal format offers lot-to-lot consistency for quantitative Western blot comparisons across experimental replicates. The vial should be stored refrigerated and protected from light; aliquoting is recommended to limit freeze-thaw cycles of the glycerol-containing formulation. This product is supplied for research use only and is not intended for diagnostic or therapeutic applications.