Anti-CREB (phospho Ser133) Antibody [B1C4]

Supplied through Antibodies.com, this lyophilised reagent is a Mouse monoclonal IgG2 antibody (clone B1C4) that selectively recognises the phosphorylated Ser133 epitope of human CREB. The immunogen corresponds to a serine-133-phosphorylated peptide fragment of human CREB conjugated to KLH, and the antibody was generated in the Sp2/0-Ag14 myeloma line before being purified by Protein G affinity chromatography. Intended exclusively for research use, the preparation is supplied as a 500 µg lyophilised cake in 0.01 M Phosphate Buffered Saline at pH 7.0, with the working concentration described as reconstitution-dependent. It is unconjugated, reacts with human samples, and is validated by the manufacturer for ELISA applications, making it well suited to phospho-CREB detection in cell-based or biochemical assays performed in vitro. Shipped on refrigerated gel packs from St. Louis, Missouri, with a typical lead time of 6–9 business days, the antibody combines the specificity of a monoclonal reagent with the convenience of a freeze-dried format, allowing laboratories to reconstitute aliquots at the concentration appropriate to their assay. Researchers working on cAMP-response element-binding protein signalling, transcriptional regulation, or kinase-pathway studies will find this reagent a reliable tool for monitoring CREB activation status via Ser133 phosphorylation, the canonical marker of CREB-dependent transcriptional activity.