Anti-CREB (phospho-S129) Antibody

Phosphorylation of the cAMP response element-binding protein (CREB) at serine 129 is a post-translational modification increasingly studied in neuronal signaling, synaptic plasticity, and transcriptional regulation. The Antibodies.com Anti-CREB (phospho-S129) Antibody is a rabbit polyclonal reagent raised against this phosphorylated epitope, supporting selective detection of the modified protein in endogenous samples.

Each vial contains 100 µl of antibody at 1 mg/ml in phosphate buffered saline (PBS) supplemented with 0.05% sodium azide at approximately pH 7.2. The reagent is unconjugated, offering flexibility to pair with user-selected secondary antibodies for chromogenic or fluorescent readout. Reactivity spans human, mouse, and rat, and the product is validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications.

In practice, researchers apply the antibody to cell or tissue lysates under standard immunoblotting conditions, or to fixed sections in IHC/IF workflows where phospho-specific recognition helps distinguish the S129-phosphorylated CREB pool from total CREB. The polyclonal format provides broader epitope coverage, which can be advantageous when probing conserved phosphorylated regions across species.