Anti-CREB-BP Antibody

This rabbit polyclonal antibody targets CREBBP (CREB-binding protein), a multifunctional transcriptional coactivator that bridges sequence-specific transcription factors with the basal transcriptional machinery and couples chromatin remodeling to gene activation. Produced by Antibodies.com as an unconjugated primary reagent in a 100 µl volume, the antibody is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human samples.

The polyclonal response recognizes epitopes across the CBP protein, supporting detection of full-length CREBBP in cell lysates and tissue sections. Because CREBBP functions as a scaffold for CREB, p53, NF-κB, and several nuclear receptors, researchers use this reagent to investigate transcriptional regulation, histone acetyltransferase activity, and signaling pathways linked to Rubinstein-Taybi syndrome and several malignancies. The unconjugated format allows flexible pairing with user-selected secondary antibodies and fluorophore or enzyme conjugates, which is useful when a custom detection scheme is required for double-labeling or amplification steps.

For long-term storage, aliquot the reagent and store frozen; avoid repeated freeze-thaw cycles to preserve binding activity. For immunofluorescence and IHC, the antibody provides a tool for examining nuclear localization of CREBBP, while Western blot experiments confirm expression across cell lines and primary tissue. Researchers should perform appropriate titration and include positive controls expressing endogenous CBP.