Anti-Creatine kinase (mitochondrial 1B) Antibody

This rabbit polyclonal antibody from Antibodies.com targets mitochondrial creatine kinase 1B (CKMT1B), the sarcomeric mitochondrial isoform that catalyzes reversible phosphotransfer between ATP and creatine to buffer cellular energy in tissues such as cardiac and skeletal muscle. Supplied as 100 µl of unconjugated reagent, it is validated for western blot and immunohistochemistry on human samples, giving researchers flexibility to pair it with their preferred secondary labels and detection chemistries.

CKMT1B localizes to the inner mitochondrial membrane, making this antibody well suited to studies of bioenergetics, muscle physiology, and mitochondrial pathology. Raised in rabbit and purified as a polyclonal reagent, it offers broad epitope coverage of the target, useful when probing denatured lysates in western blot or fixed tissue sections in immunohistochemistry. The unconjugated format further supports custom labelling workflows, including biotin, HRP, or fluorophore conjugation when a particular detection scheme is required.

Typical workflows begin with sample preparation from cultured cells or tissue homogenates, followed by SDS-PAGE separation for western blot, or deparaffinization and antigen retrieval for IHC on formalin-fixed sections. The antibody is reactive with human CKMT1B. Store refrigerated and protected from light; aliquot upon first use to minimize freeze-thaw cycles and preserve activity across multiple experiments.