Anti-COX7B Antibody

Generated in rabbit and affinity-purified, this unconjugated polyclonal reagent from Antibodies.com targets cytochrome c oxidase subunit 7B (COX7B), a nuclear-encoded component of mitochondrial Complex IV that supports electron transport and oxidative phosphorylation. Supplied as 100 µL of unconjugated antisera in a single vial, the reagent reacts with human COX7B and supports both western blot and immunohistochemistry workflows, allowing laboratories to probe mitochondrial respiratory chain composition in tissue sections and protein lysates.

For western blot, the antibody detects the COX7B polypeptide under standard denaturing and reducing conditions on PVDF or nitrocellulose membranes. In immunohistochemistry, the same reagent localizes the protein in formalin-fixed, paraffin-embedded tissue samples, supporting subcellular localization studies of mitochondrial markers. The unconjugated format makes it straightforward to pair the primary with HRP, alkaline phosphatase, or fluorophore secondary reagents chosen by the end user. Store the vial refrigerated and protected from light, aliquot on receipt to limit freeze-thaw cycles, and handle under standard laboratory cold-chain conditions.

Reported reactivity covers human samples, supporting translational work in which COX7B expression is examined in patient-derived material, cultured human cell lines, and xenograft tissue. Investigators focusing on mitochondrial dysfunction, neurodegeneration, metabolic disease, or cancer metabolism can apply this reagent to compare COX7B levels across cohorts and to confirm expression changes suggested by transcriptomic studies. The polyclonal immunoglobulin pool offers broader epitope coverage than a typical monoclonal preparation, which can be advantageous when post-translational variants or processing fragments of the target are of interest. This product is intended for research use only.