Anti-COX7A1 Antibody

Probing mitochondrial complex IV assembly in metabolically active tissues requires a reagent capable of resolving a small (~9 kDa) nuclear-encoded subunit reliably across species. The rabbit polyclonal IgG distributed by Antibodies.com fits that brief for immunoblotting: it is an unconjugated primary specific for COX7A1 (cytochrome c oxidase subunit 7A1, muscle isoform; UniProt P24310, gene ID 1346), supplied as a 100 µl liquid aliquot and validated for Western blot on both human and mouse sample sets.

Because the reagent is unconjugated, researchers can choose the detection chemistry — HRP with enhanced chemiluminescence, alkaline phosphatase, or fluorescent secondaries — to match the sensitivity each experiment needs, and the polyclonal format offers the multi-epitope recognition that often helps when a single monoclonal clone gives borderline signal on a low-abundance band in the 7-9 kDa range. A predicted molecular weight near 9 kDa means 15% or higher Tris-tricine gels and careful transfer conditions help preserve the band during blotting.

The reagent is suited to studies of oxidative phosphorylation in skeletal muscle and heart, screening patient-derived cell lines or biopsies for cytochrome c oxidase deficiency, and tracking COX7A1 expression dynamics during myogenic differentiation, cardiac stress, or exercise adaptation protocols. Cross-reactivity has been demonstrated on human and mouse material, enabling side-by-side comparison between clinical samples and murine models of mitochondrial disease.

For long-term stability, store refrigerated, protect from light, aliquot on first opening into smaller single-use volumes, and avoid repeated freeze-thaw cycles across multi-month campaigns. Intended for research use only, not for diagnostic or therapeutic applications.