Anti-COX4I1 Antibody

COX4I1 (cytochrome c oxidase subunit 4 isoform 1, mitochondrial) is a nuclear-encoded subunit of complex IV in the electron transport chain, making it a widely used loading control for mitochondrial content in western blot work. This rabbit polyclonal IgG antibody from Antibodies.com recognizes endogenous COX4I1 in human, mouse, and rat samples, supplied as 50 µl of unconjugated antisera.

The antibody has been validated across multiple research applications including western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and flow cytometry. Researchers commonly use it to normalize mitochondrial mass in lysates, to map COX4I1 distribution in tissue sections, and to immunoprecipitate intact complex IV for interaction studies. Because COX4I1 expression is generally stable across many cell types, it is often paired with other markers when comparing oxidative phosphorylation activity under different metabolic or stress conditions.

For best results, aliquot the antibody upon arrival and store at -20°C to minimize freeze-thaw cycles; protect from light. The polyclonal format increases the likelihood of recognizing multiple epitopes on the mature and precursor forms of the subunit, which is useful when probing cleaved or processed mitochondrial fractions.