Anti-COT2 Antibody

Raised in rabbit as an unconjugated polyclonal reagent, this Antibodies.com primary antibody targets COT2, the Cancer Osaka Thyroid oncoprotein also known as MAP3K8 or Tpl2. It is a serine/threonine kinase that functions as an upstream activator of the MEK/ERK and JNK branches of the MAPK signaling cascade. The reagent has been validated for Western blot and immunofluorescence and detects the endogenous protein in human, mouse, and rat samples, supporting studies of immune cell signaling, inflammatory responses downstream of TNF and IL-1 receptors, and tumor biology in cancers in which MAP3K8 contributes to proliferation, survival, or transformation.

The antibody is supplied as 100 µl of antiserum in a stabilizing buffer intended for refrigerated storage. A working aliquot can be frozen for repeated bench use while limiting freeze-thaw cycles. Researchers probing Cot/Tpl2-driven MAPK activity in lymphocytes and macrophages, or screening altered expression in tumor sections and cell lines, will find this format useful for routine blotting and for localization work by IF microscopy.