Anti-Cortisol-3 Antibody

Raised in rabbit against the cortisol-3 hapten, this polyclonal reagent from Antibodies.com is supplied as neat serum in a 1 ml vial and is unconjugated for flexible labelling in custom assay workflows. Cortisol-3 (also called cortisol-3-CMO or cortisol-3-carboxymethyloxime) is a structural derivative of the glucocorticoid hormone cortisol, conjugated at the C-3 position of the steroid ring, and antibodies raised against this immunogen are widely used as the recognition reagent in competitive immunoassays that quantify cortisol levels in research samples. The product targets the C3-derivatised cortisol epitope rather than free cortisol directly, which makes it a useful component for ELISA plate coating, competitive radioimmunoassay development, and lateral-flow test prototyping focused on steroid hormone measurement. Because the antibody is unconjugated, end users can attach their preferred reporter enzyme, fluorophore, or biotin tag using standard coupling chemistries such as NHS-ester or maleimide reactions. The polyclonal nature of the reagent provides broad epitope coverage, which can be advantageous when detecting cortisol metabolites and structurally related glucocorticoids in complex biological matrices. Storage at -20 °C in aliquots is recommended to preserve activity across multiple freeze-thaw cycles. Applications include stress physiology research, HPA-axis studies, adrenal function investigations, and veterinary endocrinology workflows where cortisol serves as a circulating biomarker. Each vial contains 1 ml of polyclonal antibody in neat serum format, ready for dilution into working buffer at the concentration appropriate for the chosen detection method.