Anti-Connexin 43 (R361) Antibody

Gap junction channels assembled from Connexin 43 (Cx43, encoded by GJA1) mediate direct intercellular coupling in excitable and epithelial tissues, and the protein is the principal substrate for PKA- and PKC-driven phosphorylation that controls channel gating. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against a synthetic peptide centred on residue R361 of the human Cx43 C-terminal cytoplasmic tail, and is validated for western blot and immunohistochemistry on human, mouse and rat samples. Supplied as a 50 µl aliquot at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, the antiserum is widely used in studies of cardiac arrhythmia, epidermal wound healing, astrocyte biology and lens fibre differentiation, where Cx43 remodelling is a hallmark of disease and repair. The C-terminal epitope at R361 lies downstream of the regulatory phosphosites Ser368, Ser279 and Ser282, enabling parallel assessment of total Cx43 alongside phospho-specific reagents on the same blot or tissue section. For best results, store refrigerated and avoid repeated freeze-thaw cycles to preserve antibody activity.