Anti-Connexin 43 Antibody

Targeting Connexin 43 (Cx43), this unconjugated polyclonal antibody from Antibodies.com recognizes a ~43 kDa gap junction channel protein encoded by the GJA1 gene. Cx43 assembles into hexameric connexons that dock between adjacent cells to permit direct intercellular exchange of ions, second messengers, and small metabolites, supporting synchronized cardiac contraction, neural signaling, and epidermal barrier function.

Raised in rabbit and supplied as 100 µl of liquid antiserum, the reagent is validated for western blot detection of the endogenous protein band, immunohistochemistry on formalin-fixed paraffin-embedded sections, immunofluorescence localization in cultured cells and tissue cryosections, and ELISA-based quantitation. Cross-reactivity is confirmed against human, mouse, and rat samples, enabling translational studies that compare Cx43 expression across species and disease models such as arrhythmogenic cardiomyopathy, ischemia-reperfusion injury, wound healing, and glioma invasion.

For best results, keep refrigerated between uses, aliquot to limit freeze-thaw cycles, and protect from light to preserve antibody activity across repeated applications.