Anti-Connexin 25 Antibody

Raised in rabbits, this unconjugated polyclonal reagent from Antibodies.com targets the human Connexin 25 gap junction protein for use in Western blot and ELISA workflows. The antibody is supplied as 100 µl of antiserum and is reactive with human samples, providing a specific detection option for laboratories investigating connexin family expression, intercellular channel communication, and membrane biology in tissues where this less-characterized isoform has been described.

Connexin 25 belongs to the beta class of gap junction proteins and assembles into hexameric hemichannels that dock between adjacent cells to permit direct transfer of ions, signaling molecules, and small metabolites. Polyclonal reagents against rarer connexin isoforms remain valuable for researchers mapping tissue-specific distribution, validating GJB7 transcript expression at the protein level, or probing channel assembly in primary cell culture models and tissue lysates.

Recommended applications include Western blot for protein-level detection in prepared lysates and ELISA for quantitative work. The unconjugated format offers flexible pairing with user-selected reporter enzymes, fluorophores, or streptavidin systems. Aliquot upon receipt and refrigerate to preserve activity across multiple freeze-thaw cycles; protect from light when handling.