Anti-COMT Antibody

This polyclonal reagent recognizes catechol-O-methyltransferase (COMT), the magnesium-dependent enzyme that transfers a methyl group from S-adenosylmethionine onto catechol substrates such as dopamine, norepinephrine, and epinephrine. Raised in rabbit, the antibody is validated for Western blot (WB) and immunocytochemistry (ICC) workflows and cross-reacts with human, mouse, and rat samples, supporting comparative studies of catecholamine metabolism in cell models and rodent tissues.

Each vial from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. The unconjugated format allows researchers to pair the reagent with their own secondary antibody, fluorophore, or enzyme conjugate. For best results, aliquot upon receipt and store frozen at -20 degrees C, avoiding repeated freeze-thaw cycles to preserve immunoreactivity across multiple experiments.

COMT is expressed as a soluble cytoplasmic isoform (S-COMT) and a membrane-bound variant (MB-COMT) generated by alternative promoter usage, and is widely investigated in the context of prefrontal cortex dopamine tone, stress responses, estrogen modulation, and pharmacogenomic variation in methyl donor pathways. The 100 ul pack size is sufficient for dozens of immunoblots and covers the slides typically needed for cell-based imaging experiments, making the reagent practical for laboratories profiling COMT expression across patient-derived samples and animal disease models.