Anti-Collagen IV Antibody

Produced by Antibodies.com, this research-grade reagent is a rabbit polyclonal antibody raised against Collagen IV and supplied as a 50 µL aliquot at 1.0 mg/mL in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a stabiliser. The antibody is unconjugated and was affinity-purified from rabbit antiserum by epitope-specific affinity chromatography, yielding a preparation of purified Rabbit IgG that detects endogenous levels of total Collagen IV protein. The immunogen is a synthesised peptide derived from human Collagen IV, and the reagent is validated for Western Blot (WB), Immunohistochemistry (IHC) and Immunocytochemistry/Immunofluorescence (ICC/IF) workflows. It has been verified to react with Human and Mouse samples, making it suitable for cross-species studies of basement-membrane biology, with the target corresponding to COL4A4 (Collagen alpha-4(IV) chain). The product is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Intended for research use only, this polyclonal reagent from Antibodies.com offers a flexible tool for laboratories investigating extracellular matrix composition, glomerular basement membrane integrity, fibrotic disease models and tumour microenvironment studies, where reliable detection of Collagen IV in tissue sections and cell preparations is required.