Anti-Collagen IV Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets Collagen IV, the major structural collagen that assembles into the sheet-like network of every vertebrate basement membrane. Supplied at 100 µl per vial, the reagent is validated for two core laboratory workflows: Western blot detection of the protein in cell and tissue lysates, and immunohistochemistry localization of Collagen IV in fixed tissue sections. Confirmed reactivity covers both human and mouse samples, supporting cross-species studies in glomerular kidney disease, vascular biology, tumor-stroma interactions, dermal and ocular fibrosis, and basement-membrane remodeling in organoid and regenerative-medicine models. The polyclonal nature of the reagent allows recognition of multiple epitopes across the Collagen IV triple-helical and non-collagenous (NC1) domains, which helps maintain signal in samples where individual epitopes may be partially masked by chemical fixation or limited proteolysis. The unconjugated format gives researchers full flexibility to pair the antibody with HRP, alkaline phosphatase, or fluorophore-conjugated secondary reagents of their choice. Each vial is supplied as a liquid stock ready for dilution into standard blocking or antibody diluent buffers, and the reagent should be refrigerated for short-term storage, with the stock divided into single-use aliquots to limit freeze-thaw cycles and preserve lot-to-lot consistency across extended studies.