Anti-Collagen III Antibody

This rabbit polyclonal antibody from Antibodies.com targets type III collagen, a fibrillar extracellular matrix protein that forms reticular fibers in skin, blood vessel walls, and internal organs. Supplied as 100 µl of unconjugated antiserum, the reagent is intended for Western blot, immunohistochemistry, and immunofluorescence applications on human samples, where it labels reticular connective tissue and fibrotic lesions.

Type III collagen (COL3A1) is well known for its role in reticular connective tissue and in early wound repair, where it is deposited before being gradually replaced by type I collagen. The polyclonal format detects multiple epitopes across the collagen III molecule, including the triple-helical and telopeptide regions, supporting visualization of both intact and partially processed matrix in tissue sections, cell-derived ECM, and conditioned culture samples. Because the reagent is unconjugated, it can be paired with HRP-, biotin-, or fluorophore-labeled secondary reagents suited to the chosen assay, including multiplexed immunofluorescence panels that distinguish type III from type I collagen distribution in fibrosis, tumor stroma, and vascular remodeling research.