Anti-Collagen II Antibody

Offered by Antibodies.com, this affinity-purified reagent is a rabbit polyclonal reagent raised against Collagen II and designed for laboratory research use. The antibody detects endogenous levels of total Collagen II and was generated using a synthesized peptide derived from the human protein as the immunogen. It is supplied at a concentration of 1.0 mg/mL in a 50 µl volume, formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. The host species is Rabbit, the clonality is Polyclonal, and the conjugate is Unconjugated, allowing flexible downstream labeling by the end user. For storage, the product is shipped at 4 °C; upon arrival it should be aliquoted and stored at –20 °C, with care taken to avoid repeated freeze/thaw cycles. Validated applications include Western blot (WB) and Immunohistochemistry (IHC), and the reagent has been shown to react with Human, Mouse, and Rat samples, making it suitable for cross-species studies of cartilage, chondrocyte biology, and extracellular matrix research. Purification by affinity-chromatography minimizes background and supports specific detection in both blotting and tissue-staining workflows. For research use only; not intended for diagnostic or therapeutic applications.