Anti-Collagen II Antibody

Type II collagen is the principal fibrillar protein of hyaline cartilage and the nucleus pulposus, encoded by the COL2A1 gene and essential for maintaining tissue resilience under mechanical load. Rabbit polyclonal antibodies raised against this target offer broad epitope coverage along the triple-helix and telopeptide regions, allowing detection of both mature fibrils and newly synthesized procollagen in cartilage biology, chondrogenesis, and osteoarthritis research workflows.

This unconjugated reagent from Antibodies.com is validated for Western blot analysis of COL2A1 protein, immunohistochemical localization in fixed tissue sections, and immunofluorescence imaging of cartilage explants, growth plates, and intervertebral disc specimens. The antibody cross-reacts with Human, Mouse, and Rat samples, enabling comparative work between patient biopsies and standard laboratory models including murine and rat cartilage.

Supplied as 100 µl of unconjugated antiserum, the polyclonal reagent fits standard indirect immunostaining protocols, including fluorophore-conjugated secondary detection and chromogenic IHC. For long-term storage, aliquot upon arrival and store at -20 degrees C, avoiding repeated freeze-thaw cycles to preserve binding activity. Each lot is quality controlled to ensure consistent signal across blotting and imaging applications.