Anti-COL9A1 Antibody

COL9A1, the alpha-1 chain of type IX collagen, is a structural component of cartilage extracellular matrix, vitreous humor, and other connective tissues, where it associates with type II collagen fibrils to maintain tissue integrity. Antibodies.com offers a rabbit polyclonal antibody raised against human COL9A1, validated for Western blot and immunohistochemistry and supplied as an unconjugated reagent at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol.

The 100 µl format provides sufficient volume for routine probing of chondrocyte lysates, articular cartilage sections, and intervertebral disc tissue. Investigators working on osteoarthritis, multiple epiphyseal dysplasia (MED), Stickler syndrome, and related connective tissue disorders use this reagent to characterize collagen IX assembly and to map COL9A1 expression across developmental and disease models. Polyclonal reactivity offers broader epitope coverage than monoclonal alternatives, an advantage when detecting processed or partially degraded forms of the approximately 92 kDa protein.

Store at -20 °C in working aliquots to minimize freeze-thaw cycles and protect from light during bench handling. The glycerol and azide preservatives support short-term stability at 4 °C for active use. For research use only.