Anti-COL5A2 Antibody

Manufactured by Antibodies.com as a primary antibody for collagen research, this rabbit polyclonal reagent is raised against COL5A2 (collagen type V alpha 2 chain) and is supplied at a working concentration of 1 mg/mL in phosphate-buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2. Each vial contains 50 µL of the unconjugated antibody, which was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen (a synthetic peptide corresponding to residues in human COL5A2), with purity greater than 95% by SDS-PAGE. The antibody is validated for use in WB (Western blot) and detects endogenous levels of COL5A2 protein, with a reported molecular weight of approximately 145 kDa. Host species is Rabbit, clonality is Polyclonal, and the conjugate is Unconjugated; recommended storage is at -20°C in small aliquots to avoid repeated freeze/thaw cycles. Reactivity has been confirmed against Human and Rat samples (with Mouse also reported in similar catalog entries), making it suitable for cross-species collagen studies. For Western blot applications, dilution ranges of approximately 1:500–1:1000 are typically recommended. The reagent is intended for research use only and is not for diagnostic or therapeutic applications. Suitable secondary antibodies such as Goat Anti-Rabbit IgG H&L conjugates are offered separately for downstream detection.