Anti-COL5A1 Antibody

Manufactured by Antibodies.com, this affinity-purified reagent is a rabbit polyclonal antibody raised against a synthetic peptide of human COL5A1 (collagen type V alpha 1) and intended for the detection of endogenous total COL5A1 protein. Supplied as a 50 µl aliquot at 1.0 mg/mL in phosphate-buffered saline (pH 7.4, without Mg²⁺ and Ca²⁺) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the preparation is unconjugated and azide-stabilised, making it suitable for downstream labelling as required. The antibody is recommended for Western blot (WB) and immunohistochemistry (IHC) workflows and has been validated for reactivity with mouse samples. The host species is rabbit, the clonality is polyclonal, and the antibody was purified by affinity chromatography against the immunogen to reduce non-specific background. For routine handling the product is shipped at 4 °C and should be aliquoted and stored at −20 °C upon arrival, with repeated freeze/thaw cycles avoided to preserve activity. As an unconjugated primary, it pairs cleanly with common anti-rabbit IgG secondary reagents, and compatibility with both chromogenic and fluorescence-based detection systems has been demonstrated. The reagent is for research use only and is not intended for diagnostic or therapeutic applications. Each lot is quality-controlled to deliver consistent performance across immunoblotting and tissue-section applications, supporting reproducible studies of collagen V biosynthesis, extracellular matrix organisation, and connective-tissue biology.