Anti-COL5A1 Antibody

This Rabbit polyclonal antibody from Antibodies.com specifically targets COL5A1, the alpha-1 chain of type V collagen, a fibrillar collagen that nucleates heterotypic fibril assembly with type I collagen in tissues including skin, tendon, placenta, and corneal stroma. Provided as an unconjugated preparation at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the antibody is validated for Western blot and immunohistochemistry workflows. The 50 µl volume supports multiple experimental runs, and the polyclonal format enables recognition of multiple epitopes across the COL5A1 polypeptide. Demonstrated reactivity with Human and Mouse samples makes this reagent useful for cross-species studies of connective tissue biology, with particular relevance to investigations of classical Ehlers-Danlos syndrome, where COL5A1 mutations disrupt fibrillogenesis. Researchers working on extracellular matrix composition, collagen deposition in fibrosis, wound healing models, and tissue morphogenesis will find the antibody adaptable to standard immunoblotting detection on PVDF or nitrocellulose membranes as well as chromogen-based IHC on fixed paraffin-embedded tissue sections.