Anti-COL4A2 Antibody

This Rabbit polyclonal reagent from Antibodies.com targets the alpha-2(IV) collagen chain encoded by the COL4A2 gene, providing broad coverage of collagen IV biology in vascular basement-membrane research. The antibody is produced in rabbit and purified by affinity-chromatography using an epitope-specific immunogen derived from the N-terminal region of human COL4A2, ensuring selective recognition of endogenous target protein. It belongs to the IgG class and is supplied in an unconjugated format at a working concentration of 1 mg/mL, formulated in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide as a preservative. Researchers can apply the antibody across a versatile panel of assays, including ELISA, Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC), making it well suited for both cell-based and tissue-based studies. Reactivity has been validated against human and mouse samples, supporting translational workflows that bridge model-organism data with clinical specimens. The 50 µl pack size is convenient for pilot optimization, while a100 µl option is available for larger studies. For handling, the antibody is shipped at 4°C and should be aliquoted upon arrival and stored long-term at -20°C, with careful avoidance of repeated freeze/thaw cycles to preserve activity. As a research-use-only polyclonal reagent from Antibodies.com, this COL4A2 antibody offers a reliable tool for probing basement-membrane composition, angiogenesis, and vascular pathology, complementing related Collagen IV investigations.