Anti-COL4A2 Antibody

Basement membrane biology depends on type IV collagen, and the Alpha 2 chain (COL4A2) is a key structural partner alongside COL4A1, COL4A3, COL4A4, COL4A5, and COL4A6. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum validated for western blot, immunohistochemistry, immunofluorescence, and ELISA against human and mouse samples.

Because COL4A2 contributes the protein backbone that anchors epithelial, endothelial, and muscle cells to underlying connective tissue, the antibody is useful for studying angiogenesis, glomerular architecture, tumor stroma, and fibrotic remodeling. Researchers probing small vessel disease, Alport-spectrum kidney pathology, and intracranial hemorrhage models frequently use anti-COL4A2 reagents to compare patient biopsies with murine tissues, since mutations in COL4A2 are linked to porencephaly and cerebral small vessel disease phenotypes.

The unconjugated format pairs cleanly with HRP, fluorescent dye, or biotin secondary reagents chosen for the detection modality. For western blot, lysates from cultured cells or snap-frozen tissue are resolved on SDS-PAGE and detected after transfer; immunohistochemistry on formalin-fixed paraffin-embedded sections benefits from citrate or EDTA antigen retrieval; immunofluorescence on cryosections or fixed cells can use Alexa Fluor or FITC conjugates downstream. Aliquot on first thaw and store the vial refrigerated between uses to limit freeze-thaw damage and preserve long-term titer.

This Antibodies.com product ships as a single 100 µl vial, supporting both pilot screening runs and larger cohort studies in translational vascular and renal research programs.