Anti-COL4A1 Antibody

Supplied through Antibodies.com, this primary polyclonal reagent is a rabbit polyclonal antibody raised against COL4A1, the gene encoding the alpha-1 chain of type IV collagen. It is presented as an unconjugated IgG at a concentration of 1 mg/ml, packaged in a 50 µl vial, and is validated for Western Blot (WB), ELISA, and Immunohistochemistry (IHC) applications. The antibody is reactive with both human and mouse samples, making it suitable for cross-species studies of basement membrane biology and extracellular matrix research. The immunogen is a synthetic peptide corresponding to residues near the C-terminal region of human COL4A1, and the host species is rabbit, ensuring a polyclonal response with broad epitope coverage. Because the reagent is unconjugated, it can be paired with a wide variety of secondary antibodies and detection chemistries depending on the experimental design. For best stability and performance, vials should be stored at -20 °C; the supplier also recommends aliquoting to avoid repeated freeze-thaw cycles. Researchers using this reagent in immunohistochemistry can expect nuclear and basement membrane staining patterns consistent with the localization of type IV collagen in epithelial and endothelial tissues. Combined with WB and ELISA compatibility, the product supports workflows ranging from qualitative tissue localization to semi-quantitative protein expression analysis, all backed by the rigorous validation standards typical of the Antibodies.com catalog.