Anti-COL2A1 Antibody

Collagen type II alpha 1 chain (COL2A1) is the structural backbone of hyaline cartilage and the nucleus pulposus, and is also a major component of the vitreous humor and inner ear. This rabbit polyclonal primary antibody from Antibodies.com is supplied as 100 µl of unconjugated antiserum for use in Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows on human, mouse, and rat samples.

The reagent targets the alpha-1(II) collagen product and supports studies of chondrocyte differentiation, articular cartilage biology, intervertebral disc degeneration, and skeletal development. In immunohistochemistry on cartilage sections, the antibody labels the pericellular and territorial matrix surrounding chondrocytes, allowing assessment of type II collagen deposition in growth plates, articular surfaces, and disc tissue. Researchers in skeletal disease and connective tissue biology pair COL2A1 staining with COL1A1, COL9A1, COL10A1, and COL11A1 markers to map the collagen composition of native and engineered cartilage constructs.

The polyclonal format provides broad epitope coverage, which is helpful for detecting splice variants such as the type IIA procollagen isoform expressed by chondroprogenitor cells. The unconjugated format allows pairing with custom HRP, biotin, or fluorophore reporters depending on the detection method. For long-term stability, aliquot the vial on arrival and store frozen, protecting the reagent from repeated freeze-thaw cycles. Each lot is generated in rabbit host and validated for specificity to the alpha-1(II) collagen chain, supporting reproducible labeling across cartilage, disc, and ocular tissues.