Anti-COL10A1 Antibody

Raised in rabbit and affinity-purified, this unconjugated polyclonal IgG reagent targets Collagen Type X Alpha 1 (COL10A1) and is supplied by Antibodies.com as a 50 µL aliquot in a single-use vial. The antibody is validated for immunohistochemistry on formalin-fixed paraffin-embedded or frozen tissue sections, and its unconjugated format allows pairing with user-selected secondary detection chemistries, including chromogenic DAB staining and immunofluorescence workflows.

Reactivity has been confirmed against human, mouse, and rat tissue, supporting comparative studies of skeletal development, fracture repair, and cartilage pathology across these species. COL10A1 encodes the alpha chain of type X collagen, a short-chain homotrimer produced specifically by hypertrophic chondrocytes during endochondral ossification and localized to the hypertrophic zone of growth plate cartilage, where it contributes to the mineralization of cartilage transitioning into bone. Researchers use this reagent to characterize chondrocyte maturation, to model osteoarthritis progression, and to investigate Schmid-type metaphyseal chondrodysplasia (SMCD) and Japanese-type spondylometaphyseal dysplasia, two skeletal disorders directly linked to COL10A1 mutations. Reports in the cancer literature have additionally highlighted COL10A1 expression within tumor stromal compartments, expanding its research applications beyond cartilage and bone biology.

For research use only.