Anti-COL10A1 Antibody

Affinity-purified polyclonal antiserum raised in rabbit against a recombinant fusion protein corresponding to amino acids 521-680 of human collagen type X (COL10A1, NP_000484.2), this unconjugated IgG reagent from Antibodies.com is supplied as 100 µl of liquid in PBS (pH 7.30) with 0.02% sodium azide and 50% glycerol. It is validated for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections from human, mouse, and rat specimens, with a recommended IHC dilution of 1:100 to 1:200. Because collagen type X is a short-chain homotrimer expressed almost exclusively by hypertrophic chondrocytes during endochondral ossification, the reagent is commonly used to map growth-plate mineralization zones, monitor chondrocyte differentiation in vitro, and characterize lesion margins in osteoarthritis, fracture repair, and Schmid-type metaphyseal chondrodysplasia (MCDS, COL10A1-related) research. The unconjugated format permits pairing with HRP, biotin, or fluorescence-labelled secondary antibodies depending on laboratory workflow. Storage recommendation: shipped at 4°C, aliquoted upon arrival and stored at -20°C, with freeze-thaw cycles avoided. For research use only.