Anti-Clock (E270) Antibody

The CLOCK protein sits at the core of the mammalian circadian oscillator, where it heterodimerizes with BMAL1 to drive transcription of Period and Cryptochrome genes that sustain ~24-hour behavioral and metabolic cycles. This polyclonal antibody, raised in rabbit against an epitope mapping near residue E270 of human CLOCK, recognizes a region in the protein's regulatory domain and has been used in published work to detect endogenous CLOCK in lysates and tissue sections across human, mouse, and rat samples.

From Antibodies.com, the reagent ships as a 50 µl volume at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. The unconjugated format is suited to western blot and immunohistochemistry workflows. Investigators studying suprachiasmatic nucleus function, peripheral clock entrainment, sleep–wake biology, and metabolic rhythms linked to circadian disruption use it to monitor CLOCK expression and post-translational regulation in cell culture and in vivo models.

For storage, keep the vial at 4 °C for short-term use; for longer intervals, freeze aliquots at -20 °C to preserve activity across multiple freeze-thaw cycles.