Anti-Cleaved-Notch 1 (V1754) Antibody

Manufactured by Antibodies.com, this affinity-purified rabbit polyclonal reagent is raised against a synthetic peptide derived from the internal region of human Notch 1 and specifically detects endogenous levels of the activated Notch 1 fragment generated by proteolytic cleavage adjacent to V1754. Supplied as 50 µl of an unconjugated liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, the antibody is validated for use in Western blot, ELISA, immunohistochemistry (IHC) and immunocytochemistry/immunofluorescence (ICC/IF) applications, and it has been confirmed to react with human, mouse and rat samples. Validation data on the Antibodies.com product page include Western blot analysis of KB cells as well as mouse brain and rat muscle lysates, supporting broad utility across common model systems. The antibody is shipped on cold packs at 4 °C and should be aliquoted upon arrival for long-term storage at −20 °C, with repeated freeze/thaw cycles avoided to preserve performance. Intended strictly for research use, this Cleaved-Notch 1 (V1754) polyclonal antibody offers a reliable tool for investigators studying Notch signalling pathway activation, T-cell leukaemia biology, and related caspase- or γ-secretase-dependent cleavage events.