Anti-Cleaved-Notch 1 (V1754) Antibody

Produced by Antibodies.com, this rabbit polyclonal reagent specifically recognizes the cleaved, signaling-competent form of Notch 1 generated when gamma-secretase cleaves the transmembrane receptor at the V1754 site. By selectively binding the Notch intracellular domain released after S3 proteolysis, the antibody lets researchers distinguish active receptor signaling from total Notch 1 expression across human, mouse, and rat samples in Western blot, immunohistochemistry, and ELISA workflows.

Validated for immunoblotting of cleaved NICD as well as chromogenic and fluorescent staining of fixed tissue sections, the unconjugated antibody ships in a 100 µl vial and supports studies of developmental cell-fate decisions, hematopoietic stem cell maintenance, T-cell lineage specification, angiogenic vascular patterning, and Notch-driven oncogenesis in tumors such as T-ALL and triple-negative breast cancer, where aberrant V1754 cleavage sustains proliferative and stemness signaling.

Use this reagent when probing for receptor activation rather than full-length precursor levels, and pair it with a total Notch 1 antibody to map the balance between membrane-bound and cleaved receptor pools in cell lysates or paraffin-embedded tissue.