Anti-Cleaved-Notch 1 (V1754) Antibody

Raised in rabbit as a polyclonal reagent, this unconjugated primary antibody from Antibodies.com is supplied in a 100 µl vial at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide, approximately pH 7.2. It recognizes the gamma-secretase–cleaved Val1754 neoepitope of human Notch1, the activating proteolytic event that releases the Notch intracellular domain (NICD) from the transmembrane precursor. Detection of this cleaved fragment is a widely used readout of canonical Notch pathway activity in studies of T-cell development, angiogenesis, and tumor cell signaling. The reagent cross-reacts with mouse and rat Notch1, supporting translational work across rodent models and human tissue specimens. Validated applications include western blot and immunohistochemistry on formalin-fixed samples. Because the antibody is unconjugated, it pairs flexibly with HRP-, biotin-, or fluorophore-conjugated secondary reagents for chromogenic, fluorescence, or chemiluminescent detection. For storage, keep refrigerated at 4 °C; aliquot to limit freeze-thaw cycles and protect from light to preserve activity over the working shelf life of the vial.