Anti-Cleaved-Lamin A (D230-) Antibody

Primary Antibodies from Antibodies.com include this rabbit polyclonal reagent raised against the caspase-generated fragment of Lamin A. The antibody specifically recognizes the cleavage event at aspartate 230 (D230), a site targeted by caspases during programmed cell death, allowing researchers to distinguish full-length Lamin A from the apoptosis-associated fragment in Western blot and immunohistochemistry workflows.

Supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated preparation is packaged as a 50 µl vial and is reactive against human, mouse, and rat samples. Because it binds only the cleaved species, the reagent is frequently used as an early marker of caspase activation in cell death studies, complementing caspase activity assays and TUNEL staining in tissue sections.

Researchers studying nuclear envelope breakdown, chemotherapy-induced apoptosis, or developmental cell elimination can use the antibody to localize and quantify the truncated Lamin A fragment in fixed cells and lysates.