Anti-Cleaved-Lamin A (D230-) Antibody

This polyclonal antibody from Antibodies.com specifically recognizes the caspase-cleaved fragment of Lamin A generated at aspartate 230, a hallmark proteolytic event during programmed cell death. By binding only the truncated fragment rather than the full-length nuclear intermediate filament, the reagent enables direct visualization of apoptotic cells in fixed tissue sections and on Western blot membranes.

Supplied as 100 µl of affinity-purified antibody at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the format is ready for dilution into standard blocking and incubation buffers. The unconjugated rabbit IgG format gives researchers flexibility in immunohistochemistry workflows, supporting both chromogenic and fluorescence detection chemistries without the need for secondary-step stripping.

Validated for Western blot and immunohistochemistry, the reagent shows reactivity with Human, Mouse, and Rat samples, supporting translational studies that bridge rodent disease models and human pathology. Store at 4 °C; the 50% glycerol cryoprotectant prevents freeze-thaw damage, so aliquoting is not required for routine short-term use.

Typical applications include tracking caspase activation in response to drug treatments, characterizing apoptosis in tumor sections, and confirming mitochondrial or death-receptor pathway engagement in cell-based assays. Researchers studying nuclear envelope disassembly, laminopathies, or chemotherapy-induced cell death will find this antibody a focused tool for distinguishing cleaved from intact Lamin A in lysates and tissue.