Anti-Cleaved-Caspase-9 (D353) Antibody

Cleaved-Caspase-9 (D353) marks the catalytically active form of caspase-9 generated when the zymogen is proteolytically processed at aspartate 353 during the intrinsic (mitochondrial) apoptosis pathway. This polyclonal rabbit antibody from Antibodies.com selectively recognizes the resulting ~37 kDa fragment, distinguishing active caspase-9 from full-length procaspase-9 in western blot, immunohistochemistry, and ELISA workflows. Validated reactivity covers mouse and rat samples, supporting cross-species studies of programmed cell death in neuronal injury, ischemia-reperfusion, oncology, and developmental biology models. The unconjugated format allows researchers to pair the reagent with their preferred secondary antibody, HRP conjugate, or fluorophore of choice. Supplied as 100 µl of antiserum and intended for research use only, this antibody is a flexible in-house tool for monitoring mitochondrial apoptotic activation in cell lysates and fixed tissue sections.