Anti-Cleaved-caspase 3 Antibody

Cleaved caspase-3 marks the active, proteolytically processed fragment of the executioner protease that drives the morphological hallmarks of programmed cell death. Raised in rabbit, this polyclonal reagent from Antibodies.com recognizes the Asp175-cleaved p17 subunit that is generated only after upstream initiator caspases activate the zymogen, making it a selective indicator of apoptotic rather than total procaspase-3 pools. Supplied at 50 µl per vial as an unconjugated preparation, the antibody has been validated on human, mouse, and rat samples in western blot and immunohistochemistry workflows, where it labels cells undergoing DNA-fragmentation-associated death in tissue sections and resolves the 17 kDa cleavage product alongside the 19 kDa pro-form on immunoblots. Laboratories studying neurodegeneration, ischemic injury, tumor response to chemotherapy, and developmental remodeling rely on this specificity to distinguish committed apoptotic cells from resting or proliferating populations in fixed paraffin and frozen specimens, and the unconjugated format allows secondary-label pairing with HRP, fluorophore, or biotin reporters chosen by the end user.