Anti-Cleaved-Caspase-2 p18 (G170) Antibody

Raised in rabbit, this unconjugated polyclonal antiserum from Antibodies.com recognizes the p18 large subunit that remains after caspase-2 has cleaved itself at the G170 site, the activation step that converts the inactive zymogen into a catalytically competent cysteine protease. Because the epitope corresponds to the cleaved fragment rather than the full-length precursor, the reagent serves as a marker for cells that have actually entered the caspase-2-dependent apoptotic pathway, not merely those expressing the pro-form.

Antibodies.com validates the reagent for Western blot and ELISA applications, with reactivity demonstrated against human samples; the 100 µL liquid aliquot is supplied ready to dilute into standard immunoblotting or plate-based workflows. Researchers studying DNA-damage-induced apoptosis, PIDDosome signaling, or stress-driven cell death use this antibody to confirm proteolytic maturation of caspase-2 in cell lysates and to compare cleaved-subunit levels across treated versus untreated cultures, providing a read-out that is specific to the active enzyme rather than total protein expression.