Anti-CLDN8 Antibody

Produced in rabbit as a polyclonal IgG and supplied unconjugated, this reagent from Antibodies.com specifically recognizes claudin-8 (CLDN8), a four-transmembrane-domain component of epithelial tight junctions that contributes to paracellular ion permeability. The antibody is validated for Western blot detection of endogenous CLDN8 in mouse and rat tissue lysates and is commonly used to characterize tight junction composition in renal epithelial models, profile claudin-8 expression in experimental kidney injury, and probe distal tubule chloride transport mechanisms.

Each order ships as a 50 µL liquid aliquot in a single vial, suitable for standard immunoblotting workflows on kidney samples. Typical working protocols involve resolving renal membrane fractions by SDS-PAGE, transferring to PVDF or nitrocellulose, and probing under reducing conditions to detect the claudin-8 immunoreactive band. Storage at -20 °C is recommended, with the preparation kept in small aliquots to limit repeated freeze-thaw cycles of the IgG reagent.

Researchers in renal physiology and epithelial biology have applied this antibody to MDCK cell systems, primary distal tubule cultures, and rodent kidney sections, where it supports mapping of claudin isoform distribution along the nephron. Its mouse and rat reactivity makes it a practical reagent for comparative studies of paracellular barrier function across common laboratory species.