Anti-CLDN3 Antibody

Claudin-3 (CLDN3) is a ~23 kDa transmembrane tight junction protein belonging to the claudin family, where it contributes to paracellular sealing and the structural integrity of epithelial and endothelial barriers. This rabbit polyclonal antibody from Antibodies.com is supplied as 100 µl of unconjugated antiserum and is validated for Western blot and immunohistochemistry workflows across human, mouse, and rat samples. In Western blot, the reagent typically yields a discrete band near the expected molecular weight of endogenous CLDN3, while on fixed tissue sections it produces membranous and junctional staining consistent with the protein's localization at apical tight junctions. Investigators apply it to characterize epithelial barrier function, probe changes in tight junction organization in cancer models where CLDN3 expression is frequently altered, and assess tissue-specific claudin composition in normal and diseased epithelia. The polyclonal format offers recognition of multiple epitopes on the target, which is useful when working with truncated isoforms or post-translationally modified claudin species. Handle the vial refrigerated and protected from light, and prepare working aliquots to limit freeze-thaw cycles.