Anti-CLCA4 Antibody

The CLCA4 protein (UniProt Q14CN2) is a calcium-activated chloride channel regulator that resides primarily on the basolateral membrane of intestinal and airway epithelial cells, where it modulates chloride conductance, mucus secretion, and barrier homeostasis. This polyclonal antibody from Antibodies.com was raised in rabbit against the human target and is provided as an unconjugated IgG in a 100 µL liquid aliquot, suitable for western blot and immunohistochemistry workflows on formalin-fixed paraffin-embedded or fresh-frozen human tissue sections.

Detection by western blot typically yields a band near the expected 120 kDa precursor mass, with cleavage products sometimes visible. In published colorectal studies, CLCA4 expression is frequently reduced in adenoma and carcinoma tissue compared with matched normal mucosa, making this reagent a useful marker for epithelial differentiation status and gut tumor biology, where loss of staining has been associated with disease progression. The unconjugated format offers flexibility to pair the primary with an HRP-, biotin-, or fluorophore-conjugated secondary of the user's choice.

Validated applications include western blot analysis of epithelial lysates and immunohistochemistry on gastrointestinal and respiratory sections, with reactivity reported against human antigen. The reagent is intended for research use only and should be stored refrigerated at 2-8 degrees C in working aliquots to limit freeze-thaw cycles of the stock solution.