Anti-Claudin 5 Antibody

Raised in rabbit and affinity-purified by chromatography, this polyclonal antibody from Antibodies.com targets the tight-junction protein Claudin 5 and is supplied at a concentration of 1.0 mg/mL in a 50 µL volume of phosphate-buffered saline (pH 7.4, 150 mM NaCl) supplemented with 0.02% sodium azide and 50% glycerol as a cryoprotectant. The reagent is unconjugated and was generated against a synthesized peptide derived from human Claudin 5, allowing it to detect endogenous levels of total Claudin 5 in samples from human, mouse, and rat origins. It is validated for Western blotting (recommended dilution range 1:500–1:3000) and immunohistochemistry, making it suitable for studies of blood–brain barrier integrity, endothelial tight junctions, and related neuroscience or vascular biology applications. The antibody is shipped at 4 °C and should be aliquoted upon arrival and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Intended for research use only and not for diagnostic or therapeutic procedures, this Antibodies.com reagent offers a reliable polyclonal option for laboratories investigating claudin family expression patterns across species.