Anti-Claudin-1 Antibody

Raised in rabbit against the tight-junction transmembrane protein Claudin-1, this unconjugated polyclonal reagent is supplied as 100 µl in a vial and is validated across western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. Cross-reactive with human, mouse, and rat samples, it supports studies of epithelial barrier integrity, paracellular permeability, and the molecular organization of cell-cell junctions in primary tissue and cultured cell models.

Claudin-1 is a ~23 kDa tetraspan membrane protein that forms the backbone of tight junction strands and is widely investigated in the context of skin barrier function, intestinal and renal epithelial transport, hepatocyte polarity, and the entry mechanisms of certain viruses that co-opt claudin family members as attachment factors. Researchers commonly deploy anti-Claudin-1 antibodies to map junction disruption in disease states such as hepatocellular carcinoma, inflammatory bowel disease, and keratinocyte disorders, and to confirm tight-junction reassembly in barrier-recovery assays.

The unconjugated presentation allows flexible pairing with user-selected secondary labels for chromogenic IHC, fluorescent IF, and chemiluminescent western blot detection. Store refrigerated, protect from light, and aliquot the stock upon arrival to minimize freeze-thaw cycles. This Antibodies.com reagent is intended for in vitro research use and pairs naturally with other tight-junction markers in co-localization studies of epithelial monolayers.