Anti-Claudin 1 Antibody

Manufactured by Antibodies.com as a rabbit polyclonal primary antibody, this affinity-purified reagent is raised against Claudin 1 and is supplied at a 50 µl volume with a working concentration of 1.0 mg/mL. Provided unconjugated in a liquid formulation of phosphate buffered saline (pH 7.4, 150 mM NaCl) containing 0.02% sodium azide and 50% glycerol as a cryoprotectant, the antibody detects endogenous levels of total Claudin 1 protein in samples derived from human, mouse, and rat origins. The host species is rabbit, the clonality is polyclonal, and the immunoglobulin class is IgG; the reagent is produced from rabbit antiserum by epitope-specific affinity chromatography using a synthesized peptide immunogen derived from human Claudin 1. Verified applications include Western blot (WB) and immunohistochemistry (IHC); suggested starting dilutions and buffer conditions are listed on the supplier data sheet. For best stability, the antibody should be stored at −20 °C and protected from repeated freeze/thaw cycles, which can compromise the affinity-purified IgG fraction. Researchers studying tight junction biology, epithelial barrier function, or related signaling pathways can use this Antibodies.com reagent for routine detection of Claudin 1 in cell lysates and tissue sections, taking advantage of the cross-reactivity profile that covers the three most commonly used mammalian model species. The product is intended for research-use only and ships on cold packs from the company's U.S. fulfillment center in St. Louis, MO, with a typical lead time of 6–9 business days; complete QC data, recommended dilutions per application, and the full lot-specific certificate of analysis are documented on the supplier page.