Anti-CKS1B Antibody

Raised in rabbit and purified by antigen affinity chromatography, this polyclonal IgG reagent from Antibodies.com binds the CDC28 protein kinase regulatory subunit 1B (CKS1B), a roughly 9 kDa adaptor of SCF ubiquitin ligase complexes that drives proteasomal turnover of the cyclin-dependent kinase inhibitor p27 and supports G1/S cell-cycle progression.

Validated for western blot detection of endogenous CKS1B in human, mouse, and rat samples, the unconjugated antibody is supplied as a 50 µl liquid volume in PBS containing 150 mM NaCl, 50% glycerol, and 0.02% sodium azide. Recommended WB working dilutions typically fall in the 1:500 to 1:1000 range, with adjustments depending on lysate preparation and chemiluminescent detection chemistry.

Investigators working in myeloma genetics, oncogenic signalling, and cell-cycle checkpoint biology use this reagent to score CKS1B levels across proliferating and arrested populations, since amplification of the CKS1B locus at chromosome 1q21 has been linked to poor prognosis in multiple myeloma and to altered p27 degradation kinetics in tumour-derived cell lines and primary tissue samples.

Shipped at 4 °C, the vial should be aliquoted upon arrival and stored at -20 °C to limit freeze-thaw cycles and preserve antibody activity over extended use. Intended for research use only.